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UV-Vis

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An overview of electromagnetic radiation absorption. This example discusses the general principle using visible light. A white beam source – emitting light of multiple wavelengths – is focused on a sample (the complementary color pairs are indicated by the yellow dotted lines). Upon striking the sample, photons that match the energy gap of the molecules present (green light in this example) are absorbed in order to excite the molecule. Other photons transmit unaffected and, if the radiation is in the visible region (400–700 nm), the sample color is the complementary color of the absorbed light. By comparing the attenuation of the transmitted light with the incident, an absorption spectrum can be obtained.

吸収分光法光の吸収から物質の正体を突き止める分析技術

吸収分光法電磁波分光分析Beer-Lambert法